Ah the infamous SBPTZ.
Martin I think Han's write-up is more generic calibration explanation, while this issue is more arcane.
A few years back, when I was using both DSS and DSLR, I read this discussion on the DSS group, which might get a bit more into the nuts and bolts:
https://groups.io/g/DeepSkyStacker/topi ... 2C76212692
I admit I still don't really get it.
Note that the situation arises only with certain calibration setups, and certain DSLR's. The SBPTZ box specifies a certain command that is sent to the DCRAW call, and thus is inapplicable to astrocam FITS.
That isn't to say that the underlying situation doesn't affect all cameras, but I believe most astrocams/drivers handle the potential issue under the hood, and of course there's no call to DCRAW to untangle the various proprietery RAW formats, which to my understanding are TIFF wrappers.
Based on that io discussion, back in the day I always checked the box when I used bias frames, which I always did as I used those in lieu of both darks and dark flats. Was that error? I have no idea.
I do recall one thread on CN where I think Mark Shelley (known astro/DSLR guru) ran some data through DSS both with and without SBPTZ checked off. I can't remember if he found a minor statistical difference in the results, but no visual difference was noticeable. Of course, I don't know if the particular calibration setup and/or specific DSLR used in that case actually triggered the need (or lack thereof) of using SBPTZ anyway.
Trying to chase down what SBPTZ actually means is no easy task. But, from the DSS experts who have these discussions, the warning was that certain (maybe rare) situations of a particular DSLR, particular calibration setup used, and also the potential concurrent use of another checkbox setting in a different DSS options window, could cause bad results.
But to this day I remain baffled whether SBPTZ is just forcing (or not forcing, who knows) either what amounts to a small offset to handle possible negative pixel values (similar to use of actual offset in astrocams), or is somehow using a synthetic bias (in the manner you can set up Siril to do). So, are we talking very low offset-type values here, or is it up near the (higher) bias or base darks value?
Note I have not yet downloaded and tried the new 5.x versions of DSS, but I don't know if it is substantively any different or has just been ported over to a new coding system.